Journal:

Article Title: An ?- E-catenin gene trap mutation defines its function in preimplantation development

doi:

Figure Lengend Snippet: β-Galactosidase expression in α-E-cateninGT1 heterozygous embryos. All plates show specimens stained for detection of β-galactosidase. (A) Whole mount E12 embryo. (B) Mesonephros and gonad stained after dissection from an E12 embryo. (C) A lung primordium from the same embryo. (D) Detail of the head region of a sagittal section of an E13 embryo after β-galactosidase staining. (E) Whole mount expanded blastocyst. e, Inner ear; eb, epithelial branches in the lung; g, gonad; icm, inner cell mass; m, mesenchyme of the lung; md, mesonephric duct; ms, mesencephalon; mt, mesonephric tubules; pmd, paramesonephric duct; so, somites; t, telencephalon; tr, trophoblast. Arrowheads in E point to the junctions between trophoblast cells. Open arrowhead in D points to the ventricular layer of the prospective telencephalic cortical region, and filled arrowhead points to the nasal epithelium.

Article Snippet: CMT cells used as controls are a mouse rectum carcinoma cell line with epithelial morphology (ATCC CCL 223).

Techniques: Expressing, Staining, Dissection

Journal: Oncology Letters

Article Title: Fatty acids inhibit anticancer effects of 5-fluorouracil in mouse cancer cell lines

doi: 10.3892/ol.2017.6190

Figure Lengend Snippet: Effect of LA or EA on 5-FU-induced reduction of cancer cell viability. CT26 cells were treated with (A) LA (50 µg/ml) or (B) EA (20 µg/ml), administered concurrently with 5-FU (1 µg/ml). LL2 cells were treated with (C) LA (50 µg/ml) or (D) EA (20 µg/ml), administered concurrently with 5-FU (1 µg/ml). CMT93 cells were treated with (E) LA (50 µg/ml) or (F) EA (20 µg/ml), administered concurrently with 5-FU (1 µg/ml). (G) LA (50 µg/ml) (H) or EA (20 µg/ml) was administered 24 h prior to 5-FU treatment (1 µg/ml) in 3 cancer cell lines. Data are expressed as the mean ± standard deviation. LA, linoleic acid; EA, elaidic acid; 5-FU, 5-fluorouracil; Mock, vehicle treated with 70% ethanol.

Article Snippet: The mouse rectal carcinoma CMT93 cell line and the mouse lung cancer LL2 cell line were purchased from DS Pharma Biomedical Co., Ltd. (Osaka, Japan).

Techniques: Standard Deviation

Journal: PLoS Neglected Tropical Diseases

Article Title: 2,4-Diaminothieno[3,2- d ]pyrimidines, a new class of anthelmintic with activity against adult and egg stages of whipworm

doi: 10.1371/journal.pntd.0006487

Figure Lengend Snippet: Summary of the cytotoxicity in a mouse epithelial cell line of the DATP series. Mouse CMT-93 rectal epithelial cells were used for this assay. Maximum tested concentration was 100 μM. n = 8, error range (in parentheses) shows 95% confidence interval. EC 50 values in the adult Trichuris paralysis assay are shown for comparison.

Article Snippet: The mouse rectal epithelial cell line CMT-93 (LGC Promochem, Teddington, United Kingdom) was used for these studies.

Techniques: Concentration Assay, Comparison